CLEC4A ELISA Kit, Human

Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.

CLEC4A ELISA Kit, Human: 製品情報

製品名
CLEC4A ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Lysate
感度
13.18 pg/mL
線形範囲
78.125-5000 pg/mL
特異性
Recognizes both recombinant and natural Human CLEC4A
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,003.00 1,980.00 3,517.00
SD 53.32 71.99 174.06
CV(%) 5.3% 3.6% 4.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 964.00 1,963.00 3,851.00
SD 121.16 221.82 364.46
CV(%) 12.6% 11.3% 9.5%
回収率
The recovery of Human CLEC4A spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
92
Range
91 -95%
直線性
Supernatant
  Recovery of detected
1:2 88%
1:4 100%
1:8 104%
1:16 96%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This CLEC4A ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CLEC4A protein in Lysate . It contains recombinant Human CLEC4A, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CLEC4A ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

CLEC4A ELISA Kit, Human: 別名

CD367 ELISA Kit, Human; CLEC4A ELISA Kit, Human; CLECSF6 ELISA Kit, Human; DCIR ELISA Kit, Human; DDB27 ELISA Kit, Human; HDCGC13P ELISA Kit, Human; LLIR ELISA Kit, Human

CLEC4A 背景情報

Dendritic cell immunoreceptor (DCIR), also known as C-type lectin domain family 4 member A (CLEC4A), C-type lectin superfamily member 6 (CLECSF6), is a single-pass type II C-type lectin receptor expressed mainly in dendritic cells (DCs), which is a negative regulator of DC expansion and has a crucial role in maintaining the homeostasis of the immune system. The Dectin-2 family of C-type lectins that includes Dectin-2, BDCA-2, DCIR, DCAR, Clecsf8 and Mincle. These type II receptors contain a single extracellular carbohydrate recognition domain and have diverse functions in both immunity and homeostasis. DCIR is the only member of the family which contains a cytoplasmic signalling motif and has been shown to act as an inhibitory receptor, while BDCA-2, Dectin-2, DCAR and Mincle all associate with FcRgamma chain to induce cellular activation, including phagocytosis and cytokine production. Dectin-2 and Mincle have been shown to act as pattern recognition receptors for fungi, while DCIR acts as an attachment factor for HIV. In addition to pathogen recognition, DCIR has been shown to be pivotal in preventing autoimmune disease by controlling dendritic cell proliferation. DCIR expressed on antigen presenting cells and granulocytes and acts as an inhibitory receptor via an intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM). It may also be involved via its ITIM motif in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation. Additionally, DCIR can participate in the capture of HIV-1 and promote infection in trans and in cis of autologous CD4(+) T cells from human immature monocyte-derived DCs. DCIR acts as a ligand for HIV-1 and is involved in events leading to productive virus infection.
完全な名称
C-type lectin domain family 4 member A
参考文献
  • Kanazawa N, et al. (2004) Signaling and immune regulatory role of the dendritic cell immunoreceptor (DCIR) family lectins: DCIR, DCAR, dectin-2 and BDCA-2. Immunobiology. 209(1-2): 179-90.
  • Fujikado N, et al. (2008) Dcir deficiency causes development of autoimmune diseases in mice due to excess expansion of dendritic cells. Nat Med. 14(2): 176-80.
  • Lambert AA, et al. (2008) The C-type lectin surface receptor DCIR acts as a new attachment factor for HIV-1 in dendritic cells and contributes to trans- and cis-infection pathways. Blood. 112(4): 1299-307.
  • Graham LM, et al. (2009) The Dectin-2 family of C-type lectins in immunity and homeostasis. Cytokine. 48(1-2): 148-55.
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