CD48 ELISA Kit, Human

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CD48 ELISA Kit, Human: 製品情報

製品名
CD48 ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Serum
感度
2.1 pg/mL
線形範囲
9.38-600 pg/mL
特異性
Recognizes both recombinant and natural Human CD48
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 147.00 261.00 501.00
SD 4.59 5.61 21.54
CV(%) 3.1% 2.1% 4.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 151.00 256.00 487.00
SD 11.41 17.55 46.85
CV(%) 7.5% 6.8% 9.6%
回収率
The recovery of Human CD48 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
107
Range
99 -115%
Sample
supernatant (n=3)
Average % Recovery
103
Range
99 -107%
直線性
Serum
  Recovery of detected
1:1 94%
1:2 100%
1:4 106%
1:8 112%
Supernatant
  Recovery of detected
1:1 80%
1:2 94%
1:4 106%
1:8 118%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This CD48 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD48 protein in Serum . It contains recombinant Human CD48, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD48 ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD48. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross -reactivity. No cross-reactivity was observed.

CD48 ELISA Kit, Human: 別名

BCM1 ELISA Kit, Human; BLAST ELISA Kit, Human; Blast-1 ELISA Kit, Human; BLAST1 ELISA Kit, Human; hCD48 ELISA Kit, Human; mCD48 ELISA Kit, Human; MEM-102 ELISA Kit, Human; SLAMF2 ELISA Kit, Human

CD48 背景情報

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
完全な名称
CD48 molecule
研究分野
参考文献
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
  • The Asthma-associated PER1-like domain-containing protein 1 (PERLD1) Haplotype Influences Soluble Glycosylphosphatidylinositol Anchor Protein (sGPI-AP) Levels in Serum and Immune Cell Proliferation
    Author
    Sio, YY;Anantharaman, R;Lee, SQE;Matta, SA;Ng, YT;Chew, FT;
    Year
    2020
    Journal
    Sci Rep
    Application
    ELISA
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