CD3 epsilon/CD3e ELISA Kit, Human

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CD3 epsilon/CD3e ELISA Kit, Human: 製品情報

製品名
CD3 epsilon/CD3e ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Serum
感度
23.24 pg/mL
線形範囲
23.44-1500 pg/mL
特異性
Recognizes both recombinant and natural Human CD3 epsilon/CD3e
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 73.98 141.59 277.02
SD 4.87 5.04 5.12
CV(%) 6.6% 3.6% 1.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 75.58 154.32 278.75
SD 4.37 8.83 9.77
CV(%) 5.8% 5.7% 3.5%
回収率
The recovery of Human CD3E spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
79
Range
74-83%
直線性
Serum
  Recovery of detected
1:2 105%
1:4 121%
1:8 126%
1:16 103%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Sample Dilution Buffer Concentrate
7. Color Reagent A
8. Color Reagent B
9. Stop Solution
製品概要
This CD3 epsilon/CD3e ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD3 epsilon/CD3e protein in Serum . It contains recombinant Human CD3 epsilon/CD3e, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD3 epsilon/CD3e ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD3E. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD3 epsilon/CD3e ELISA Kit, Human: 別名

CD3 epsilon ELISA Kit, Human; IMD18 ELISA Kit, Human; T3E ELISA Kit, Human; TCRE ELISA Kit, Human

CD3 epsilon/CD3e 背景情報

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.
完全な名称
CD3e molecule, epsilon (CD3-TCR complex)
Related Pathways
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
参考文献
  • Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
  • Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
  • Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
  • Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.
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