CD3 epsilon/CD3e ELISA Kit, Human

Temporarily not available outside of China.

CD3 epsilon/CD3e ELISA Kit, Human: 製品情報

CD3 epsilon/CD3e ELISA Kit, Human
Solid Phase Sandwich ELISA (quantitative)
23.24 pg/mL
23.44-1500 pg/mL
Recognizes both recombinant and natural Human CD3 epsilon/CD3e
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 73.98 141.59 277.02
SD 4.87 5.04 5.12
CV(%) 6.6% 3.6% 1.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 75.58 154.32 278.75
SD 4.37 8.83 9.77
CV(%) 5.8% 5.7% 3.5%
The recovery of Human CD3E spiked to different levels throughout the range of the assay in related matrices was evaluated.
serum (n=3)
Average % Recovery
  Recovery of detected
1:2 105%
1:4 121%
1:8 126%
1:16 103%
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Sample Dilution Buffer Concentrate
7. Color Reagent A
8. Color Reagent B
9. Stop Solution
This CD3 epsilon/CD3e ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD3 epsilon/CD3e protein in Serum . It contains recombinant Human CD3 epsilon/CD3e, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
This ELISA Kit is shipped at ambient temperature.
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD3 epsilon/CD3e ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD3E. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD3 epsilon/CD3e ELISA Kit, Human: 別名

CD3 epsilon ELISA Kit, Human; IMD18 ELISA Kit, Human; T3E ELISA Kit, Human; TCRE ELISA Kit, Human

CD3 epsilon/CD3e 背景情報

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.
CD3e molecule, epsilon (CD3-TCR complex)
Related Pathways
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
  • Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
  • Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
  • Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
  • Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.
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