CD200 ELISA Kit, Human

Temporarily not available outside of China.Online Datasheet is a general version, for batch specific information please refer to the hard copy in shipping package.

CD200 ELISA Kit, Human: 製品情報

製品名
CD200 ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Recombinant protein
感度
30.3 pg/mL
線形範囲
62.5-4000 pg/mL
特異性
Recognizes both recombinant and natural Human CD200
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 283.00 602.00 966.00
SD 10.11 30.02 50.01
CV(%) 2.6% 2.9% 3.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 254.00 582.00 943.00
SD 20.55 20.36 90.68
CV(%) 6.7% 3.2% 9.7%
回収率
The recovery of Human CD200 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
supernatant (n=3)
Average % Recovery
84
Range
81 -87%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
直線性
Serum
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
Supernatant
  Recovery of detected
1:2 93%
1:4 98%
1:8 109%
1:16 95%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This CD200 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD200 protein in Recombinant protein . It contains recombinant Human CD200, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD200 ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant Human CD200. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD200 ELISA Kit, Human: 別名

MOX1 ELISA Kit, Human; MOX2 ELISA Kit, Human; MRC ELISA Kit, Human; OX-2 ELISA Kit, Human; OX2 ELISA Kit, Human

CD200 背景情報

CD200 (OX-2) is a cell surface glycoprotein that imparts immune privileges by suppressing alloimmune and autoimmune responses through its receptor, CD200R, expressed primarily on myeloid cells. Signals delivered through the CD200:CD200R axis have been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells. The role of CD200-CD200R signaling in immune regulation of the central nervous system has become a popular field of research in recent years. Many studies have shown that there is a close correlation between CD200-CD200R, microglia activation, and Parkinson's disease (PD). The ability of CD200 to suppress myeloid cell activation is critical for maintaining normal tissue homeostasis but may also enhance the survival of migratory neoplastic cells. CD200 and CD200R associate via their respective N-terminal Ig-like domains. CD200 has been characterized as an important immunoregulatory molecule, increased expression of which can lead to decreased transplant rejection, autoimmunity, and allergic disease. Elevated CD200 expression has been reported to be associated with poor prognosis in some human malignancies. Besides, CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
完全な名称
Cd200 molecule
参考文献
  • Minas K, et al. (2006) Is the CD200/CD200 receptor interaction more than just a myeloid cell inhibitory signal? Crit Rev Immunol. 26(3): 213-30.
  • Wang XJ, et al. (2007) CD200-CD200R regulation of microglia activation in the pathogenesis of Parkinson's disease. J Neuroimmune Pharmacol. 2(3): 259-64.
  • Wong KK, et al. (2010) The role of CD200 in immunity to B cell lymphoma. J Leukoc Biol. 88(2): 361-72.
  • Estrogen-regulated CD200 inhibits macrophage phagocytosis in endometriosis
    Author
    Weng, L;Hou, S;Lei, S;Peng, H;Li, M;Zhao, D;
    Year
    2020
    Journal
    Journal of Reproductive Immunology
    Application
    ELISA
  • Reduced Serum Levels of Cluster of Differentiation 200 in Alcohol-Dependent Patients
    Author
    Chaturvedi, A;Rao, G;Praharaj, SK;Guruprasad, KP;Pais, V;
    Year
    2020
    Journal
    Alcohol Alcohol.
    Application
    Elisa
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