DDR1 Protein, Mouse, Recombinant (His & GST Tag)

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DDR1 Protein, Mouse, Recombinant (His & GST Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
The specific activity was determined to be 2 nmol/min/mg using synthetic modified AXLtide peptide (modified-CKKSRGDYMTMQIG) as substrate.
タンパク質の構築
A DNA sequence encoding the mouse DDR1 (Q03146-2) (Leu444-Val874) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
NCBI Reference No.
発現ホスト
Baculovirus-Insect Cells
Mouse
予測N末端
Met
分子量
The recombinant mouse DDR1/GST chimera consists of 668 amino acids and has a calculated molecular mass of 75.8kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
バッファー
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM DTT
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

DDR1 Protein, Mouse, Recombinant (His & GST Tag): 画像

DDR1 Protein, Mouse, Recombinant (His & GST Tag): 別名

6030432F18 Protein, Mouse; AI323681 Protein, Mouse; Cak Protein, Mouse; CD167a Protein, Mouse; Nep Protein, Mouse; PTK3A Protein, Mouse

DDR1 背景情報

Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 1 (MCK1), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK1/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK1/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
完全な名称
discoidin domain receptor tyrosine kinase 1
参考文献
  • Hou G, et al. (2001) The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair. J Clin Invest. 107(6): 727-35.
  • Ongusaha PP, et al. (2003) p53 induction and activation of DDR1 kinase counteract p53-mediated apoptosis and influence p53 regulation through a positive feedback loop. EMBO J. 22(6): 1289-301.
  • Jönsson M, et al. (2001) Repression of Wnt-5a impairs DDR1 phosphorylation and modifies adhesion and migration of mammary cells. J Cell Sci. 114(11): 2043-53.
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