The plasmid is confirmed by full-length sequencing.
Stable or Transient mammalian expression
保存 & 配送
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
TST cDNA ORF Clone, Mouse, C-HA tag: 別名
Rhodanese cDNA ORF Clone, Mouse
Thiosulfate sulfurtransferase (TST, EC 18.104.22.168), also known as Rhodanese, is a mitochondrial enzyme that catalyzes the transfer of sulfur in several molecular pathways. The TST gene, located on 22q12.3, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, C.elegans, and frog. This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. Also, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. TST is widely expressed in the colon, liver, and other tissues. TST deficiency was shown to be strongly related to the pathophysiology of metabolic diseases including diabetes and obesity.
thiosulfate sulfurtransferase (rhodanese)
Włodek P, et al. (2010) Thiol levels, protein carbonylation and anaerobic sulfur metabolism in erythrocytes of peritoneal dialysis and predialysis patients. Nephrology (Carlton) 15 (8): 755-761.
Libiad M, et al. (2015) Polymorphic variants of human rhodanese exhibit differences in thermal stability and sulfur transfer kinetics. J Biol Chem 290 (39): 23579-23588.
Kruithof PD, et al. (2020) Unraveling the role of thiosulfate sulfurtransferase in metabolic diseases. Biochim Biophys Acta Mol Basis Dis 1866 (6): 165716.