NEDD4 cDNA ORF Clone, Mouse, N-GFPSpark® tag

NEDD4 cDNA ORF Clone, Mouse, N-GFPSpark® tag: 製品情報

遺伝子
Mouse
NCBI 参考シーケンス番号
遺伝子の長さ
2664 bp
製品の特徴
Full length Clone DNA of Mouse neural precursor cell expressed, developmentally down-regulated 4 with N terminal GFPSpark tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
タグシーケンス
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

NEDD4に関するより多くの情報

Entrez Gene ID

NEDD4 cDNA ORF Clone, Mouse, N-GFPSpark® tag: 別名

AA959633 cDNA ORF Clone, Mouse; AL023035 cDNA ORF Clone, Mouse; AU019897 cDNA ORF Clone, Mouse; E430025J12Rik cDNA ORF Clone, Mouse; mKIAA0093 cDNA ORF Clone, Mouse; Nedd4-1 cDNA ORF Clone, Mouse; Nedd4a cDNA ORF Clone, Mouse

NEDD4 背景情報

Neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4) is an E3 ligase that degrades multiple different proteins and plays an oncogenic role in human cancer. The Nedd4 family E3 ligases are key regulators of cell growth and proliferation and are often misregulated in human cancers and other diseases. The ligase activities of Nedd4 E3s are tightly controlled via auto-inhibition. Nedd4 deficiency promoted mouse death from sepsis and cell pyroptosis, resulting from non-canonical inflammasome activation. Furthermore, Nedd4 induced the K48-linked polyubiquitination and subsequent degradation of caspase-11 through the 26S proteasome. Meanwhile, caspase-11 (or caspase-4) reciprocally regulated the level of Nedd4 protein by cleavage. Thus, Nedd4 appears to have a key role in balancing the level of non-canonical inflammasome activation in response to gram-negative bacterial infection.
完全な名称
neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
Related Pathways
  • Notch Signaling
    Notch Signaling
参考文献
  • Zhu K, et al. (2017) Allosteric auto-inhibition and activation of the nedd4 family e3 ligase itch. EMBO Rep 18 (9): 1618-1630.
  • Zhang J, et al. (2019) Diosgenin inhibits the expression of nedd4 in prostate cancer cells. Am J Transl Res 11 (6): 3461-3471.
  • Liu Q, et al. (2019) E3 ubiquitin ligase nedd4 is a key negative regulator for non-canonical inflammasome activation. Cell Death Differ 26 (11): 2386-2399.
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