MCP-1/CCL2 cDNA ORF Clone, Mouse, C-GFPSpark® tag

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MCP-1/CCL2 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 製品情報

遺伝子
Mouse
NCBI 参考シーケンス番号
遺伝子の長さ
447 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Mouse chemokine (C-C motif) ligand 2 with C terminal GFPSpark tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI (6kb + 1.18kb)
タグシーケンス
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

MCP-1/CCL2 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MCP-1/CCL2 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.

MCP-1/CCL2 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 別名

AI323594 cDNA ORF Clone, Mouse; HC11 cDNA ORF Clone, Mouse; JE cDNA ORF Clone, Mouse; MCAF cDNA ORF Clone, Mouse; MCP-1 cDNA ORF Clone, Mouse; MCP1 cDNA ORF Clone, Mouse; Scya2 cDNA ORF Clone, Mouse; Sigje cDNA ORF Clone, Mouse; SMC-CF cDNA ORF Clone, Mouse

MCP-1/CCL2 背景情報

Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection [7–9]. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.
完全な名称
chemokine (C-C motif) ligand 2
研究分野
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Vásquez-Loarte T, Trubnykova M, Guio H. Genetic association meta-analysis: a new classification to assess ethnicity using the association of MCP-1 -2518 polymorphism and tuberculosis susceptibility as a model. BMC Genetics. 2015;16:128.
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