CD59a cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag

1/1
Price:
Size:
Number:

CD59a cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 製品情報

遺伝子
Mouse
NCBI 参考シーケンス番号
遺伝子の長さ
411 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 48G/C not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Mouse CD59 antigen with C terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI(6kb+0.41kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

CD59a cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD59a cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 検証済の画像

CD59a cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 別名

AA987121 cDNA ORF Clone, Mouse; Cd59 cDNA ORF Clone, Mouse; protectin cDNA ORF Clone, Mouse; RP24-297H17.1 cDNA ORF Clone, Mouse

CD59a 背景情報

Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins.It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 8 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.
完全な名称
CD59a antigen
参考文献
  • Huang Y, et al. (2006) Defining the CD59-C9 binding interaction. J Biol Chem. 281 (37): 27398-404.
  • Sawada R, et al. (1990) Isolation and expression of the full-length cDNA encoding CD59 antigen of human lymphocytes. DNA Cell Biol. 9(3): 213-20.
  • Philbrick WM, et al. (1990) The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility. Eur J Immunol. 20(1): 87-92.
カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください U.S.A.