CD16/FCGR3 cDNA ORF Clone, Mouse, C-GFPSpark® tag
CD16/FCGR3 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 製品情報
遺伝子
種
Mouse
NCBI 参考シーケンス番号
遺伝子の長さ
1515 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Mouse Fc receptor, IgG, low affinity III with C terminal GFPSpark tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI(6kb+1.52kb)
タグシーケンス
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
CD16/FCGR3 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
CD16/FCGR3 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 検証済の画像
CD16/FCGR3 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 別名
CD16 cDNA ORF Clone, Mouse
CD16/FCGR3 背景情報
Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
完全な名称
Fc receptor, IgG, low affinity III
研究分野
参考文献
- Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
- Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
- Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
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