Calreticulin cDNA ORF Clone, Mouse, untagged

1/1
Price:
Size:
Number:

Calreticulin cDNA ORF Clone, Mouse, untagged: 製品情報

遺伝子
Mouse
NCBI 参考シーケンス番号
遺伝子の長さ
1251 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 348T/C, 969C/T not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Mouse calreticulin.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
HindIII + XbaI (6.1kb + 1.25kb)
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Ampicillin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

Calreticulin cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Calreticulin cDNA ORF Clone, Mouse, untagged: 検証済の画像

Calreticulin cDNA ORF Clone, Mouse, untagged: 別名

Calregulin cDNA ORF Clone, Mouse; CRT cDNA ORF Clone, Mouse

Calreticulin 背景情報

Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum, calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.
完全な名称
calreticulin
参考文献
  • Michalak M, et al. (2002) Calreticulin in cardiac development and pathology. Biochim Biophys Acta. 1600(1-2):32-7.
  • Chao MP, et al. (2010) Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47. Sci Transl Med. 2(63):63ra94.
  • Andrin, C, et al. (1998) Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-cell granules. Biochemistry. 37(29):10386-94.
カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください U.S.A.