The plasmid is confirmed by full-length sequencing.
保存 & 配送
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
ADH5 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
Carbonic anhydrases IX (CAIX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide ( H2O + CO2 = H+ + HCO3- ) and thus participate in a variety of biological and physical processes. CAIX is a transmembrane protein structurally consisting of a signal peptide, a proteoglycan-related region, a CA domain with a highly conserved active site, a transmembrane anchor and an intracytoplasmic tail, and is the only tumor-associated CA isoenzyme known so far. Compared with normal tissues, CAIX is overexpressed in a wide spectrum of tumor types and associated with increased metastasis and poor prognosis in aggressive carcinomas. CAIX expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. CA9 is regarded as a new therapeutic target for CA9-derived carcinomas.
alcohol dehydrogenase 5 (class III), chi polypeptide
Pastorek, J. et al., 1994, Oncogene. 9: 2877-88.
Opavsky, R. et al., 1996, Genomics. 33: 480-7.
Swietach, P. et al., 2008, J. Biol. Chem. 283: 20473-83.
Robertson, N. et al., 2004, Cancer. Res. 64: 6160-5.
Bui, M.H. et al., 2003, Clin. Cancer. Res. 9: 802-11.
Choi, S.W. et al., 2008, Hum. Pathol. 39: 1317-22.