NEDD4 cDNA ORF Clone, Human, C-Myc tag

NEDD4 cDNA ORF Clone, Human, C-Myc tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
2703 bp
製品の特徴
Full length Clone DNA of Human neural precursor cell expressed, developmentally down-regulated 4
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
タグシーケンス
Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

NEDD4 cDNA ORF Clone, Human, C-Myc tag: 検証済の画像

NEDD4 cDNA ORF Clone, Human, C-Myc tag: 別名

NEDD4-1 cDNA ORF Clone, Human; RPF1 cDNA ORF Clone, Human

NEDD4 背景情報

Neural precursor cell expressed developmentally down-regulated protein 4 (NEDD4) is an E3 ligase that degrades multiple different proteins and plays an oncogenic role in human cancer. The Nedd4 family E3 ligases are key regulators of cell growth and proliferation and are often misregulated in human cancers and other diseases. The ligase activities of Nedd4 E3s are tightly controlled via auto-inhibition. Nedd4 deficiency promoted mouse death from sepsis and cell pyroptosis, resulting from non-canonical inflammasome activation. Furthermore, Nedd4 induced the K48-linked polyubiquitination and subsequent degradation of caspase-11 through the 26S proteasome. Meanwhile, caspase-11 (or caspase-4) reciprocally regulated the level of Nedd4 protein by cleavage. Thus, Nedd4 appears to have a key role in balancing the level of non-canonical inflammasome activation in response to gram-negative bacterial infection.
完全な名称
neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
Related Pathways
  • Notch Signaling
    Notch Signaling
参考文献
  • Zhu K, et al. (2017) Allosteric auto-inhibition and activation of the nedd4 family e3 ligase itch. EMBO Rep 18 (9): 1618-1630.
  • Zhang J, et al. (2019) Diosgenin inhibits the expression of nedd4 in prostate cancer cells. Am J Transl Res 11 (6): 3461-3471.
  • Liu Q, et al. (2019) E3 ubiquitin ligase nedd4 is a key negative regulator for non-canonical inflammasome activation. Cell Death Differ 26 (11): 2386-2399.
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