MMP-2 cDNA ORF Clone, Human, C-OFPSpark® tag

1/1
Price:
Size:
Number:

MMP-2 cDNA ORF Clone, Human, C-OFPSpark® tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
1983 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 750 C>T, 1149 T>C, 1380 G>A, 1806 C>T not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Human matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type I V collagenase), transcript variant 1 with C terminal OFPSpark / RFP tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
HindIII + XbaI (6kb + 2.67kb)
タグシーケンス
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

MMP-2 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MMP-2 cDNA ORF Clone, Human, C-OFPSpark® tag: 検証済の画像

MMP-2 cDNA ORF Clone, Human, C-OFPSpark® tag: 別名

CLG4 cDNA ORF Clone, Human; CLG4A cDNA ORF Clone, Human; MMP-2 cDNA ORF Clone, Human; MMP-II cDNA ORF Clone, Human; MONA cDNA ORF Clone, Human; TBE-1 cDNA ORF Clone, Human

MMP-2 背景情報

Matrix Metalloproteinase-2 (MMP-2) is an enzyme that degrades components of the extracellular matrix and thus plays a pivotal role in cell migration during physiological and pathological processes. MMP-2 expression is dependent on extracellular matrix metalloproteinase inducer (EMMPRIN), Her2/neu, growth factors, cytokines, and hormones. Pro-MMP-2 activation needs MT1-MMP and TIMP-2 contribution. MMP-2 is changed in distribution and increased in amount in the ventral cochlear nucleus after unilateral cochlear ablation. A low level of MMP-2 is linked to favorable prognosis in patients with a hormone receptor-negative tumor, usually associated with high risk. As a zymogen requiring proteolytic activation for catalytic activity, MMP-2 has been implicated broadly in the invasion and metastasis of many cancer model systems, including human breast cancer (HBC). Blocking MMP-2 secretion and activation during breast carcinoma development may decrease metastasis. The detection of active MMP-2 alone or the rate of pro-MMP-2 and active MMP-2 is considered a very sensitive indicator of cancer metastasis. Modulation of MMP-2 expression and activation through specific inhibitors and activators may thus provide a new mechanism for breast cancer treatment.
完全な名称
matrix metallopeptidase 2
研究分野
参考文献
  • Thompson EW, et al. (1994) Collagen induced MMP-2 activation in human breast cancer. Breast Cancer Res Treat. 31(2-3): 357-70.
  • Jezierska A, et al. (2009) Matrix metalloproteinase-2 involvement in breast cancer progression: a mini-review. Med Sci Monit. 15(2): RA32-40.
  • Fredrich M, et al. (2010) MMP-2 is involved in synaptic remodeling after cochlear lesion. Neuroreport. 21(5): 324-7.
カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください U.S.A.