Integrin beta 3 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag

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Integrin beta 3 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
2367 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1029A/G not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Human integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) with N terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
HindIII + XbaI (6kb + 2.38kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Integrin beta 3 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Integrin beta 3 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

Integrin beta 3 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: 別名

BDPLT16 cDNA ORF Clone, Human; BDPLT2 cDNA ORF Clone, Human; CD61 cDNA ORF Clone, Human; GP3A cDNA ORF Clone, Human; GPIIIa cDNA ORF Clone, Human; GT cDNA ORF Clone, Human

Integrin beta 3 背景情報

Integrins containing the beta(3) subunit are key players in tumor growth and metastasis.ITGB3 plays an important role in ROS-induced migration and invasion in Colorectal cancer (CRC).ITGB3 is translationally activated in hypoxia and regulates malignant features, including epithelial-mesenchymal transition and cell migration, through the TGF-beta pathway, revealing a novel angle for the treatment of therapy-resistant hypoxic tumours.Bioinformatic algorithms and luciferase reporter assays revealed that integrin beta3 (ITGB3) is a direct target of miR-3a-5p.
完全な名称
integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
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