GAPDH cDNA ORF Clone, Human, C-HA tag

GAPDH cDNA ORF Clone, Human, C-HA tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
1050 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 381T/C,885T/C not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Human glyceraldehyde-3-phosphate dehydrogenase with C terminal HA tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + NotI(6kb+1.05kb)
タグシーケンス
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

GAPDH cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

GAPDH cDNA ORF Clone, Human, C-HA tag: 検証済の画像

GAPDH cDNA ORF Clone, Human, C-HA tag: 別名

G3PD cDNA ORF Clone, Human; GAPD cDNA ORF Clone, Human; HEL-S-162eP cDNA ORF Clone, Human

GAPDH 背景情報

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
完全な名称
glyceraldehyde-3-phosphate dehydrogenase
研究分野
参考文献
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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