FBXW7 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag
FBXW7 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: 製品情報
遺伝子
種
Human
NCBI 参考シーケンス番号
遺伝子の長さ
2163 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Human F-box and WD repeat domain containing 7 with N terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI(6kb+2.16kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
FBXW7 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
FBXW7 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: 検証済の画像
The plasmid was transfected into 293FT adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
FBXW7 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: 別名
AGO cDNA ORF Clone, Human; CDC4 cDNA ORF Clone, Human; FBW6 cDNA ORF Clone, Human; FBW7 cDNA ORF Clone, Human; FBX30 cDNA ORF Clone, Human; FBXO30 cDNA ORF Clone, Human; FBXW6 cDNA ORF Clone, Human; hAgo cDNA ORF Clone, Human; hCdc4 cDNA ORF Clone, Human; SEL-10 cDNA ORF Clone, Human; SEL10 cDNA ORF Clone, Human
FBXW7 背景情報
完全な名称
F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase
研究分野
Related Pathways
-
Notch Signaling
FBXW7 cDNA ORFクローン, Human, 関連製品
If you couldn't find the specific citation, you can:
