ER alpha cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag
ER alpha cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: 製品情報
遺伝子
種
Human
NCBI 参考シーケンス番号
遺伝子の長さ
1788 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Human estrogen receptor 1 with C terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + ApaI (6kb + 1.83kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
ER alpha cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
ER alpha cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: 検証済の画像
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
ER alpha cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: 別名
ER cDNA ORF Clone, Human; Era cDNA ORF Clone, Human; ESR cDNA ORF Clone, Human; ESRA cDNA ORF Clone, Human; ESTRR cDNA ORF Clone, Human; NR3A1 cDNA ORF Clone, Human
ER alpha 背景情報
An ESR1 gene mutation, which encodes for the estrogen receptor (ER) protein, is one of the potential mechanisms of therapy resistance. The ESR1 mutations result in conformational changes in the ER leading to subsequent estrogen-independent transcriptional activity. The mutations within the estrogen receptor (ER) gene, ESR1, frequently occur in metastatic breast cancer and influence response to hormone therapy. Estrogen receptor alpha (ERalpha) is one of the estrogen hormone-activated transcription factors, which regulates a large number of genes and is involved in the mammary gland development. Expression of ERalpha is considered to be a good indicator for endocrine therapy and breast cancer survival. Loss of ERalpha in breast cancer patients indicates invasiveness and poor prognosis. ESR1 as a direct target of miR-301a-3p and suggest that miR-301a-3p likely contributes to development of estrogen independence, which leads to a more invasive phenotype of breast cancer. Polymorphisms of the estrogen receptor gene alpha (ESR1) are consistently been associated with bone mineral density (BMD) and fracture.
完全な名称
estrogen receptor 1
Related Pathways
-
MAPK-Erk Pathway
ER alpha cDNA ORFクローン, Human, 関連製品
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