E-Cadherin cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag

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E-Cadherin cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
2649 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for the point mutation 2076 T/C not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) with C terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
HindIII + XbaI (6kb + 2.7kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

E-Cadherin cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

E-Cadherin cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

E-Cadherin cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: 別名

Arc-1 cDNA ORF Clone, Human; CD324 cDNA ORF Clone, Human; CDH1 cDNA ORF Clone, Human; CDHE cDNA ORF Clone, Human; E-cad cDNA ORF Clone, Human; E-Cadherin cDNA ORF Clone, Human; ECAD cDNA ORF Clone, Human; LCAM cDNA ORF Clone, Human; UVO cDNA ORF Clone, Human

E-Cadherin 背景情報

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
完全な名称
cadherin 1, type 1, E-cadherin (epithelial)
Related Pathways
  • Canonical Wnt Pathway
    Canonical Wnt Pathway
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
参考文献
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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