CSF1R cDNA ORF Clone, Human, C-HA tag

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CSF1R cDNA ORF Clone, Human, C-HA tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
2961 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 726C/T not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Human colony stimulating factor 1receptor (CSF1R) with C terminal HA tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI(two restriction sites) + XbaI(6kb+1.7kb+1.27kb)
タグシーケンス
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

CSF1R cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CSF1R cDNA ORF Clone, Human, C-HA tag: 検証済の画像

CSF1R cDNA ORF Clone, Human, C-HA tag: 別名

C-FMS cDNA ORF Clone, Human; CD115 cDNA ORF Clone, Human; CSF-1R cDNA ORF Clone, Human; CSFR cDNA ORF Clone, Human; FIM2 cDNA ORF Clone, Human; FMS cDNA ORF Clone, Human; HDLS cDNA ORF Clone, Human; M-CSF-R cDNA ORF Clone, Human; MCSF Receptor cDNA ORF Clone, Human

CSF1R 背景情報

M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation. However, studies have demonstrated that CSF1 and CSF1R have additional roles in mammary gland development during pregnancy and lactation. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.
完全な名称
colony stimulating factor 1 receptor
参考文献
  • Sherr CJ. (1990) The colony-stimulating factor 1 receptor: pleiotropy of signal-response coupling. Lymphokine Res. 9(4): 543-8.
  • Kacinski BM. (1997) CSF-1 and its receptor in breast carcinomas and neoplasms of the female reproductive tract. Mol Reprod Dev. 46(1): 71-4.
  • Sapi E, et al. (1999) The role of CSF-1 in normal and neoplastic breast physiology. Proc Soc Exp Biol Med. 220(1): 1-8.
  • Sapi E. (2004) The role of CSF-1 in normal physiology of mammary gland and breast cancer: an update. Exp Biol Med (Maywood). 229(1): 1-11.
  • Bonifer C, et al. (2008) The transcriptional regulation of the Colony-Stimulating Factor 1 Receptor (csf1r) gene during hematopoiesis. Front Biosci. 13: 549-60.
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