COX-2 cDNA ORF Clone, Human, N-His tag

COX-2 cDNA ORF Clone, Human, N-His tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
1857 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Human prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) with N terminal His tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI(6kb+1.86kb)
タグシーケンス
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

COX-2 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

COX-2 cDNA ORF Clone, Human, N-His tag: 検証済の画像

COX-2 cDNA ORF Clone, Human, N-His tag: 別名

COX-2 cDNA ORF Clone, Human; COX2 cDNA ORF Clone, Human; GRIPGHS cDNA ORF Clone, Human; hCox-2 cDNA ORF Clone, Human; PGG/HS cDNA ORF Clone, Human; PGHS-2 cDNA ORF Clone, Human; PHS-2 cDNA ORF Clone, Human

COX-2 背景情報

PTGS2, also known as COX-2, is s component of Prostaglandin-endoperoxide synthase (PTGS). PTGS, also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. PTGS2 is overexpressed in many cancers. The overexpression of PTGS2 along with increased angiogenesis and GLUT-1 expression is significantly associated with gallbladder carcinomas. Furthermore the product of COX-2, PGH2 is converted by prostaglandin E2 synthase into PGE2, which in turn can stimulate cancer progression. Consequently inhibiting COX-2 may have benefit in the prevention and treatment of these types of cancer. PTGS2 is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. It mediates the formation of prostaglandins from arachidonate and may have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.
完全な名称
prostaglandin-endoperoxide synthase 2
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Picot, et al. (1994) The X-ray crystal structure of the membrane protein prostaglandin H2 synthase-1. Nature. 367(6460):243-9.
  • Xie W, et al. (1991) Expression of a Mitogen-Responsive Gene Encoding Prostaglandin Synthase is Regulated by mRNA Splicing. Proceedings of the National Academy of Sciences. 88(7):2692-6.
  • Hla T, et al. (1992) Human Cyclooxygenase-2 cDNA. Proceedings of the National Academy of Sciences. 89(16):7384-8.
  • Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease
    Author
    Lee, JY;Han, SH;Park, MH;Baek, B;Song, IS;Choi, MK;Takuwa, Y;Ryu, H;Kim, SH;He, X;Schuchman, EH;Bae, JS;Jin, HK;
    Year
    2018
    Journal
    Nat Commun
    Application
    expression
  • N-AS-triggered SPMs are direct regulators of microglia in a model of Alzheimer's disease
    Author
    Lee, JY;Han, SH;Park, MH;Song, IS;Choi, MK;Yu, E;Park, CM;Kim, HJ;Kim, SH;Schuchman, EH;Jin, HK;Bae, JS;
    Year
    2020
    Journal
    Nat Commun
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