Bikunin/AMBP cDNA ORF Clone, Human, C-OFPSpark® tag

Bikunin/AMBP cDNA ORF Clone, Human, C-OFPSpark® tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
1059 bp
製品の特徴
Full length Clone DNA of Human alpha-1-microglobulin/bikunin precursor with C terminal OFPSpark / RFP tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
タグシーケンス
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

Bikunin/AMBP cDNA ORF Clone, Human, C-OFPSpark® tag: 別名

A1M cDNA ORF Clone, Human; EDC1 cDNA ORF Clone, Human; HCP cDNA ORF Clone, Human; HI30 cDNA ORF Clone, Human; IATIL cDNA ORF Clone, Human; ITI cDNA ORF Clone, Human; ITIL cDNA ORF Clone, Human; ITILC cDNA ORF Clone, Human; UTI cDNA ORF Clone, Human

Bikunin/AMBP 背景情報

The AMBP [A1M (alpha1-microglobulin)/bikunin precursor] gene encodes two plasma glycoproteins: A1M, an immunosuppressive lipocalin, and bikunin, a member of plasma serine proteinase inhibitor family with prototypical Kunitz-type domain. Although previously believed to be constitutively expressed exclusively in liver, the present study demonstrates the induction of this gene by oxalate in porcine proximal tubular LLC-PK1 cells and rat kidney. In liver, the precursor protein is cleaved in the Golgi network by a furin-like enzyme to release constituent proteins, which undergo glycosylation before their export from the cell. In the renal tubular cells, A1M and bikunin co-precipitate, indicating lack of cleavage of the precursor protein. As the expression of the AMBP gene is regulated by A1M-specific cis elements and transcription factors, A1M protein was studied as a representative of AMBP gene expression in renal cells. The alpha(1)-microglobulin/bikunin precursor (AMBP) gene, and its two protein products were studied in mouse embryos of 8.5-15.5 days of embryonic development by in situ hybridization and immunohistochemistry. AMBP mRNA is strongly transcribed in liver parenchyma, pancreas, and intestine epithelium. Sites of weaker expression are the vessels of the umbilical cord, the developing vertebral bodies, and kidney. The alpha(1)-microglobulin and bikunin proteins are accordingly present in developing hepatocytes, pancreas, kidney, and gut. However, additional sites of protein distribution were found that do not correlate to mRNA localization: alpha(1)-microglobulin was found in myocytes and bikunin in cardiac muscle, nervous system microvasculature, and connective tissue
完全な名称
alpha-1-microglobulin/bikunin precursor
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