Influenza A H7N9 (A/Anhui/1/2013) Matrix protein 1(Codon Optimized) Gene ORF cDNA clone expression plasmid, C-OFPSpark tag

1/2
Price:
Size:
Number:

Influenza A H7N9 (A/Anhui/1/2013) Matrix protein 1(Codon Optimized) Gene ORF cDNA clone expression plasmid, C-OFPSpark tag: 製品情報

遺伝子
H7N9
遺伝子の長さ
759 bp
シーケンスの特徴
A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in E.coli cell system.The translated amino acid sequence is identical with EPI439506.
製品の特徴
Full length Clone DNA of Influenza A H7N9 (A/Anhui/1/2013) Matrix protein 1 with C terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI (6kb + 0.81kb)
タグシーケンス
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Matrix protein 1/M1 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Influenza A H7N9 (A/Anhui/1/2013) Matrix protein 1(Codon Optimized) Gene ORF cDNA clone expression plasmid, C-OFPSpark tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

Citation List

Matrix protein 1/M1 背景情報

Influenza virus matrix protein 1 (M1) has been shown to play a crucial role in the virus replication, assembly and budding.The matrix protein 1 (M1) is the most abundant structural protein in influenza A virus particles.Matrix protein 1 (M1) of the influenza A virus plays multiple roles in virion assembly and infection. M1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the M1 vaccine.
完全な名称
cholinergic receptor, muscarinic 1

Matrix protein 1/M1 cDNA ORFクローン, H1N1, 関連製品

カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください U.S.A.