CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His Tag)

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CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His Tag): 製品情報

純度
(43.8+13.1+41.9) % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA.
Immobilized human CD3D & CD3E Heterodimer Protein (Cat:CT038-H2508H) at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 7.0-18.0 ng/ml.
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Phe 22 & Asp 23
分子量
The recombinant heterodimer of human CD3D&CD3E comprises 272 (126+146) amino acids and has a calculated molecular mass of 31.2 (14.4+16.8)KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His Tag): 画像

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His Tag): 別名

CD3-DELTA Protein, Human; IMD19 Protein, Human; T3D Protein, Human

CD3D & CD3E Heterodimer 背景情報

完全な名称
CD3d molecule, delta (CD3-TCR complex)
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