PKM2 Proteins, cDNA Clones Research Reagents

PKM (Pyruvate Kinase M1/2) is a protein coding gene located on human chromosome 15q23. PKM is also known as PK3, TCB, p58, OIP3, PKM2, CTHBP, THBP1 and HEL-S-30. The human PKM gene encodes a 57937 Da protein containing 531 amino acids. The PKM protein is ubiquitously expressed in brain, esophagus and other tissues. Among its related pathways are Glucose metabolism and Photodynamic therapy-induced HIF-1 survival signaling. PKM is related to MHC class II protein complex binding. PKLR is an important paralog of PKM gene. PKM is associated with some diseases, including Pyruvate Kinase Deficiency Of Red Cells and Pontocerebellar Hypoplasia, Type 10.

PKM2 Protein (2)

    PKM2 cDNA Clone (90)

    NM_002654.3

    クローニングベクター cDNA 製品

    In lentiviral vector

    NM_011099.3

    クローニングベクター cDNA 製品

    In lentiviral vector

    NM_001253883.1

    クローニングベクター cDNA 製品

    In lentiviral vector

    NM_053297.2

    クローニングベクター cDNA 製品

    In lentiviral vector

    XM_005559965.1

    クローニングベクター cDNA 製品

    In lentiviral vector

    NM_001195645.1

    クローニングベクター cDNA 製品

    In lentiviral vector

    PKM2 qPCR Primer (2)

    PKM2 Lysate (1)

      PKM2 の背景知識

      Pyruvate kinase isozymes M2 also known as pyruvate kinase muscle isozyme 2 (PKM2), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an isoenzyme of the glycolytic enzyme pyruvate kinase. Pyruvate kinase isozymes M2 / PKM2 is a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. PKM2 may stimulate POU5F1-mediated transcriptional activation. This protein plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms of PKM2 contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

      PKM2 の参考文献

      • Bluemlein K, et al. (2011) No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis. Oncotarget. 2 (5): 393-400.
      • Gupta V, et al. (2010) Dominant Negative Mutations Affect Oligomerization of Human Pyruvate Kinase M2 Isozyme and Promote Cellular Growth and Polyploidy. J Biol Chem. 285 (22): 16864-73.
      • Eigenbrodt E, et al. (1992) Double role for pyruvate kinase type M2 in the expansion of phosphometabolite pools found in tumor cells. Crit Rev Oncog. 3 (1): 91-115.

      Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.