PDE6D cDNA Clones Research Reagents

PDE6D (Phosphodiesterase 6D, also known as PDED; JBTS22), located on 2q37.1, is a Protein Coding gene. This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. The gene produces a 17420 Da protein composed of 150 amino acids. PDE6D is a phosphodiesterase that binds to prenyl groups and has a critical role in ciliogenesis. The C. elegans C27H5.1 polypeptide shares approximately 70% amino acid similarity with the human PDE6D protein. Diseases such as Joubert Syndrome 22 and Orofaciodigital Syndrome Vi are associated with PDE6D.

PDE6D cDNA Clone (18)

NM_001291018.1

In expression vector

NM_001108806.1

クローニングベクター cDNA 製品

In lentiviral vector

NM_001194606.1

クローニングベクター cDNA 製品

In lentiviral vector

PDE6D の背景知識

PrBP/δ, encoded by the Pde6d gene, is an isoprenyl-binding protein that regulates trafficking of isoprenylated proteins, such as PDE6 and GRK1, from photoreceptor inner segments to outer segments. The human gene (PDE6D) encoding a new subunit (delta) has been characterized and mapped to the long arm of chromosome 2 (HSA2q35-q36) where a new autosomal recessive retinitis pigmentosa (arRP) locus (RP26) has been localized. Characterization of the canine PDE6D shows the gene is about 4.2kb containing four exons interrupted by three introns. Overexpression of PDE6D in mouse eyes caused elevated intraocular pressure (IOP), and this elevation was reversed by topical ocular application of either anecortave acetate (AA) or anecortave desacetate (AdesA). The identification of PDE6D as the molecular binding partner of AA provides insight into the role of this drug candidate in treating glaucoma.

PDE6D の参考文献

  • Zhang H, et al. (2014) Unc119 gene deletion partially rescues the grk1 transport defect of pde6d (- /-) cones. Adv Exp Med Biol 801 487-493.
  • Zhang H, et al. (2007) Deletion of prbp/delta impedes transport of grk1 and pde6 catalytic subunits to photoreceptor outer segments. Proc Natl Acad Sci U S A 104 (21): 8857-8862.
  • Wang W, et al. (1999) Molecular characterization and mapping of canine cgmp-phosphodiesterase delta subunit (pde6d). Gene 236 (2): 325-332.
  • Shepard AR, et al. (2013) Identification of pde6d as a molecular target of anecortave acetate via a methotrexate-anchored yeast three-hybrid screen. ACS Chem Biol 8 (3): 549-558.

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