PCNA Proteins, Antibodies, cDNA Clones Research Reagents

PCNA (Proliferating Cell Nuclear Antigen) is a protein coding gene located on human chromosome 20p12.3. PCNA is also known as ATLD2. The human PCNA gene encodes a 28769 Da protein containing 261 amino acids. The PCNA protein is broadly expressed in bone marrow, lymph node and other tissues. Among its related pathways are Transcription-Coupled Nucleotide Excision Repair (TC-NER) and Telomere C-strand (Lagging Strand) Synthesis. PCNA is related to identical protein binding and damaged DNA binding. PCNA is associated with some diseases, including Ataxia-Telangiectasia-Like Disorder 2 and Bowen's Disease.

PCNA Protein (1)

    PCNA Antibody (3)

      PCNA cDNA Clone (45)

      NM_002592.2

      クローニングベクター cDNA 製品

      In lentiviral vector

      NM_011045.2

      クローニングベクター cDNA 製品

      In lentiviral vector

      NM_001266090.1

      クローニングベクター cDNA 製品

      In lentiviral vector

      PCNA Lysate (1)

        PCNA の背景知識

        Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.

        PCNA の参考文献

        • Balajee AS, et al. (2001) Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acids Res. 29 (6): 1341-51.
        • Ducoux M, et al. (2001) Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta. J Biol Chem. 276 (52): 49258-66.
        • Tetsuo I, et al. (2002) PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA. Genes Cells. 7(10): 997-1007.

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