|Comments for pCMV3-SP-N-HA:|
|• CMV promoter: bases 250-837
• Enhancer: bases 838-1385
• SV40 early promoter: bases 2327-2696
• Hygromycin ORF: bases 2714-3739
• pUC origin: bases 4382-5055
• Kanamycin ORF: bases 5129-5944
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constitutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
|Sequencing Primer||Forward: T7(TAATACGACTCACTATAGGG)； Reverse: BGH(TAGAAGGCACAGTCGAGG)|
pCMV3-SP-N-HA is recommended for constructing the N-HA tag secretary and membrane proteins expression vector which containing a naïve signal peptide. An universal signal peptide is used to instead the naïve signal peptide.
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3'. The amino acid sequence is: YPYDVPDYA.