pCMV3-C-OFPSpark®
pCMV3-C-OFPSpark® Physical Map
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Comments for pCMV3-C-OFPSpark®: |
| • CMV promoter: bases 250-837 • Enhancer: bases 838-1385 • SV40 early promoter: bases 2987-3356 • Hygromycin ORF: bases 3374-4399 • pUC origin: bases 5042-5715 • Kanamycin ORF: bases 5789-6604 |
Cloning Sites for ORF Inserting
Expression Vector pCMV3-C-OFPSpark® Information Description
| Vector Name | pCMV3-C-OFPSpark® |
| Vector Size | 6746bp |
| Vector Type | Mammalian Expression Vector |
| Expression Method | Constitutive, Stable / Transient |
| Promoter | CMV |
| Antibiotic Resistance | Kanamycin |
| Selection In Mammalian Cells | Hygromycin |
| Protein Tag | OFPSpark® |
| Sequencing Primer | Forward: T7(TAATACGACTCACTATAGGG)； Reverse: BGH(TAGAAGGCACAGTCGAGG) |
OFPSpark® Tag Information
OFPSpark® is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566 nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark® is more than twice brighter than mOrange2. Fast OFPSpark® maturation makes it clearly detectable in mammalian cells as early as within 8 hrs after transfection.
OFPSpark® can be expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with OFPSpark® expression vectors produce bright fluorescence in 8 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. For its monomer structure, OFPSpark® performs well in some fusions and protein labeling applications.
