NLK/Nemo Like Kinase Proteins, Antibodies, cDNA Clones Research Reagents

NLK (Nemo Like Kinase), located on 17q11.2, is a Protein Coding gene. The gene produces a 58283 Da protein composed of 527 amino acids. NLK contributes to various signaling pathways via its ability to interact with and modulate the activities of diverse transcription factors, including TCF/LEF proteins, SOX11, HMG2L1, and STAT3. NLK contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, and MAP kinase subfamily. The related pathways of NLK include Signaling by GPCR and Regulation of activated PAK-2p34 by proteasome-mediated degradation.

NLK/Nemo Like Kinase Protein (1)

    NLK/Nemo Like Kinase Antibody (1)

      NLK/Nemo Like Kinase cDNA Clone (15)

      NM_016231.4

      クローニングベクター cDNA 製品

      In lentiviral vector

      NLK/Nemo Like Kinase qPCR Primer (1)

      NLK/Nemo Like Kinase Lysate (1)

        NLK/Nemo Like Kinase の背景知識

        Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, Nemo-like kinase regulates some transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1, and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.

        NLK/Nemo Like Kinase の参考文献

        • Smit L, et al. (2004) Wnt activates the Tak1/Nemo-like kinase pathway. J Biol Chem. 279(17): 17232-40.
        • Yasuda J, et al. (2004) Nemo-like kinase suppresses a wide range of transcription factors, including nuclear factor-kappaB. Cancer Sci. 95(1):52-7.
        • Yasuda J, et al. (2003) Nemo-like kinase induces apoptosis in DLD-1 human colon cancer cells. Biochem Biophys Res Commun. 308(2):227-335.
        • Ishitani T, et al. (2003) Regulation of lymphoid enhancer factor 1/T-cell factor by mitogen-activated protein kinase-related Nemo-like kinase-dependent phosphorylation in Wnt/beta-catenin signaling. Mol Cell Biol. 23(4):1379-89.

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