NARFL Antibodies, cDNA Clones Research Reagents

CIAO3 (Cytosolic Iron-Sulfur Assembly Component 3, also known as PRN; HPRN; IOP1; NAR1; LET1L; NARFL), located on 16p13.3, is a Protein Coding gene. It is ubiquitously expressed in the kidney, prostate, and other tissues. The encoded protein belongs to the NARF family. CIAO3 is a component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of the iron-sulfur cluster into extramitochondrial Fe/S proteins. Site-directed mutagenesis data suggested a structural role for the C-terminal [4Fe-4S] cluster of the CIAO3 protein.

NARFL Antibody (1)

    NARFL cDNA Clone (31)

    NM_001304799.1

    In expression vector

    NM_026238.4

    クローニングベクター cDNA 製品

    In lentiviral vector

    NM_001013183.1

    クローニングベクター cDNA 製品

    In lentiviral vector

    NARFL qPCR Primer (1)

    NARFL の背景知識

    The CIAO3 protein operates at a crossroad of the cytosolic iron-sulfur protein assembly (CIA) machinery. Although the functional role of CIAO3 has been recently characterized, a description of its interaction network is still not complete. Literature data suggested that CIAO3 interacts individually with CIA2A and CIAO1 protein, with the latter two interacting with each other. However, no experimental data are available yet showing the formation of a possible ternary complex composed of CIAO3, CIAO1, and CIA2A. This work shows, for the first time, via size exclusion chromatography coupled with multiangle light scattering, UV-vis absorption, and electron paramagnetic resonance (EPR) spectroscopies, the formation of a stable, [4Fe-4S]-bound, complex, composed by CIAO3 and the hetero-CIA2A-CIAO1 complex. Moreover, site-directed mutagenesis data suggested a structural role for the C-terminal [4Fe-4S] cluster of the CIAO3 protein.

    NARFL の参考文献

    • Song D, et al. (2008) A role for iop1 in mammalian cytosolic iron-sulfur protein biogenesis. J Biol Chem 283 (14): 9231-9238.
    • Maione V, et al. (2020) Ciao3 protein forms a stable ternary complex with two key players of the human cytosolic iron-sulfur cluster assembly machinery. J Biol Inorg Chem 25 (3): 501-508.
    • Liu HZ, et al. (2017) A novel mutation in nuclear prelamin a recognition factor-like causes diffuse pulmonary arteriovenous malformations. Oncotarget 8 (2): 2708-2718.

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