MOB4A/MOB1B Proteins, Antibodies, cDNA Clones Research Reagents

MOB1B (MOB Kinase Activator 1B, also known as MATS2; MOB4A; MOBKL1A), located on 4q13.3, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, A.thaliana, rice, and frog. The gene produces a 25091 Da protein composed of 216 amino acids. The protein encoded by this gene is similar to the yeast Mob1 protein. Diseases such as Wilson-Turner X-Linked Mental Retardation Syndrome and Myeloproliferative Syndrome, Transient are associated with MOB1B. The related pathways of MOB1B include Signaling by GPCR and Signaling by Hippo.

MOB4A/MOB1B Protein (1)

    MOB4A/MOB1B Antibody (3)

      MOB4A/MOB1B cDNA Clone (15)

      BC038112

      クローニングベクター cDNA 製品

      In lentiviral vector

      MOB4A/MOB1B qPCR Primer (1)

      MOB4A/MOB1B の背景知識

      MST1 and MST2 are the mammalian Ste2-related protein kinases most closely related to Drosophila Hippo, a major regulator of cell proliferation and survival during development. Overexpression of MST1 or MST2 in mammalian cells is proapoptotic. MST1 and MST2 activity increase during mitosis, especially in nocodazole-arrested mitotic cells, where these kinases exhibit an increase in both abundance and activation. MST1 and MST2 also can be activated nonphysiologically by okadaic acid or H2O2. The MOB1B and MOBKL1B polypeptides, homologs of the Drosophila MATS polypeptide, are identified as preferred MST1/MST2 substrates in vitro and are phosphorylated in cells in an MST1/MST2-dependent manner in mitosis and response to okadaic acid or H2O2. MST1/MST2-catalyzed MOB1B/MOBKL1B phosphorylation alters the ability of MOB1B/MOBKL1B to bind and regulate downstream targets such as the NDR-family protein kinases. Thus, MOB1B/MOBKL1B phosphorylation in cells promotes MOB1B/MOBKL1B binding to the LATS1 kinase and enables H2O2-stimulated LATS1 activation loop phosphorylation. Most importantly, the replacement of endogenous MOB1B/MOBKL1B by a non-phosphorylatable mutant is sufficient to accelerate cell proliferation substantially by speeding progression through G1/S as well as mitotic exit.

      MOB4A/MOB1B の参考文献

      • Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5.
      • Gerhard DS, et al. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.
      • Devroe E, et al. (2004) Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. J Biol Chem. 279(23):24444-51.
      • Praskova M, et al. (2008) MOB1B/MOBKL1B phosphorylation by MST1 and MST2 inhibits cell proliferation. Curr Biol. 18(5):311-21.

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