MAD2L1 Proteins, Antibodies, cDNA Clones Research Reagents

MAD2L1 (Mitotic Arrest Deficient 2 Like 1, also known as MAD2; HSMAD2), located on 4q27, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog. The gene produces a 23510 Da protein composed of 205 amino acids. MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Diseases such as Nasopharyngeal Carcinoma and Breast Cancer are associated with MAD2L1.

MAD2L1 Protein (1)

    MAD2L1 Antibody (1)

      MAD2L1 cDNA Clone (15)

      NM_002358.3

      クローニングベクター cDNA 製品

      In lentiviral vector

      MAD2L1 の背景知識

      Mitotic spindle assembly checkpoint protein MAD2A, also known as HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1, and MAD2, is a nucleus and cytoplasm protein that belongs to the MAD2 family. MAD2L1 is a component of the spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase-promoting complex by sequestering CDC2 until all chromosomes are aligned at the metaphase plate. MAD2L1 has two highly different native conformations, an inactive open conformation that cannot bind CDC2 and that predominates in cytosolic monomers, and an active closed conformation. MAD2L1 in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed-form and thereby promotes interaction with CDC2.

      MAD2L1 の参考文献

      • Luo X., et al., 2000, Nat. Struct. Biol. 7:224-229.
      • Sironi L., et al., 2002, EMBO J. 21:2496-2506.
      • Mapelli M., et al., 2007, Cell 131:730-743.
      • Ho C.-Y., et al., 2008, J. Cell. Biochem. 105:835-846.
      • Skinner J.J., et al., 2008, J. Cell Biol. 183:761-768.

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