KLH Antibodies Research Reagents

Keyhole limpet hemocyanin (KLH) is a high molecular weight, heavily glycosylated oxygen-carrying metalloprotein. It is isolated from the hemolymph of Megathura crenulata and is highly immunogenic due to its structure and phylogenetic distance from mammalian proteins. KLH is composed of five subunits. It is rich in lysine and contains a large number of available primary amines to facilitate conjugation, antibody production, and promote peptide attachment after dissociation. KLH is one of the most commonly used carriers in the conjugation of antigens for antibody production.

KLH Antibody (2)

    KLH の背景知識

    KLH (Keyhole Limpet Hemocyanin) is a copper-containing oxygen carrier occurring freely dissolved in the hemolymph of many mollusks and arthropods. KLH is used extensively as a carrier protein in the production of antibodies for research, biotechnology, and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins, and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production. KLH is the most widely employed carrier proteins for this purpose. It is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and the abundance of lysine residues for coupling haptens, allows a high hapten: carrier protein ratio increasing the likelihood of generating hapten-specific antibodies. KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory. A high-quality KLH preparation with a clear opalescent blue color is the best indicator of KLH solubility. KLH is being tested as a therapeutic vaccine for a variety of cancers, including non-Hodgkins lymphoma, cutaneous melanoma, breast, and bladder cancer. These vaccines use specific tumor-associated antigens (Haptens) conjugated to KLH to stimulate the body’s immune system to generate anti-tumor immune responses which can destroy tumor cells.

    KLH の参考文献

    • Lamm, D.L. et al., 1993, Urol Res. 21 (1): 33-7.
    • Lamm, D.L. et al., 1993, J Urol. 149 (3): 648-52.
    • Jennemann, R. et al.,1994, J Biochem. 115 (6): 1047-52.
    • Harris, J.R. et al., 1999, Micron. 30 (6): 597-623.

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