To evaluate the extent of the ELISA kit results close to actual values.
A high or low recovery results in false positive or negative result affecting the accuracy of the measured value.
Make sure ELISA kit can detect natural samples and determine its applicable types of samples.
If the types of natural sample are not confirmed, a false positive or negative result may be obtained.
To evaluate the specificity of the ELISA kit and determine the binding ability of other molecules to the paired antibodies.
High cross-reactivity stands for low specificity.
To determine the effects of other molecules to the interaction of antigens with paired antibodies.
High interference stands for low accuracy of the measured value.
To ensure the stability of the ELISA kit for long-term storage.
Better stability of the ELISA kit means longer storage time.
To guarantee if similar results can be obtained when the same samples are repeatedly measured.
Poor precision of the kit will result in poor reproducibility when the same sample is repeatedly measured.
To determine the sensitivity of the ELISA kit.
Lower limit of detection stands for higher sensitivity.
To assess the accuracy of the ELISA kit.
High or low results of linear test will produce false positive or negative data.
IL 4標的 | Sino Biological Inc.社のELISA キット | 某ハイエンドブランド | 某ミドルエンドブランド | 某ローエンドブランド |
標準曲線 | 10.9-700pg/ml | 31.2 - 2,000 pg/mL | 10.24-400pg/mL | 3.12-200ng/ml |
検出限界 | 2.5pg/ml | 10 pg/mL | 2pg/mL | 0.78ng/ml |
精度 | アッセイ内またはアッセイ間のCVは15%以下 | アッセイ内またはアッセイ間のCVは15%以下 | アッセイ内またはアッセイ間のCVは15%以下 | -- |
回収率 | 80-120% | 80-120% | 80-120% | -- |
線形試験 | 80-120% | 80-120% | 80-120% | -- |
天然サンプル試験 | 細胞培養上清 | 細胞培養上清 | -- | -- |
交差反応性 | 37種類の標的 | 32 種類の標的 | 7 種類の標的 | -- |
干渉試験 | 37種類の標的 | 32 種類の標的 | 7 種類の標的 | -- |
価格 | ![]() ![]() |
![]() ![]() ![]() ![]() |
![]() ![]() ![]() |
![]() ![]() |
Th9 cell | Th2 cell | T-reg cell | Th22 cell | Th17 cell | Th1 cell | Tfh cell |
IL-10 (KIT10947A) | IL-4 (KIT11846) IL-10 (KIT10947A) |
IL-10 (KIT10947A) TGF β (KIT10804) |
CCL17 (KIT10233) TNF α (KIT10602) |
IL-17A (KIT12047) GM-CSF (KIT10015) TNF α (KIT10602) |
TNF α (KIT10602) | IL-10 (KIT10947A) IL-12 (KITCT011) IL-4 (KIT11846) |
Th9 cell | Th2 cell | T-reg cell | Th22 cell |
IL-10 (KIT10947A) | IL-4 (KIT11846) IL-10 (KIT10947A) |
IL-10 (KIT10947A) TGF β (KIT10804) |
CCL17 (KIT10233) TNF α (KIT10602) |
Th17 cell | Th1 cell | Tfh cell | |
IL-17A (KIT12047) GM-CSF (KIT10015) TNF α (KIT10602) |
TNF α (KIT10602) | IL-10 (KIT10947A) IL-12 (KITCT011) IL-4 (KIT11846) |
標的 | 線形範囲(pg/ml) | 天然サンプル試験 | 品番 |
Human CXCL9 / MIG | 15.63-1000 | Human serum | KIT10888 |
Human Fractalkine / CX3CL1 | 7.82-500 | Human milk | KIT10636 |
Human GM-CSF/CSF2 | 7.81-500 | Cell culture supernates | KIT10015 |
Human IL10/Interleukin-10 | 18.75-1200 | Cell culture supernates | KIT10947 |
Human IL-12 (IL12A & IL12B Heterodimer) | 39.07-2500 | Cell culture supernates | KITCT011 |
Human IL13/ALRH | 4.69-300 | Cell culture supernates | KIT10369 |
Human IL17A/IL17 | 4.6-300 | Cell culture supernates | KIT12047 |
Human IL32 | 62.5-4000 | Human serum | KIT11064 |
Human IL4 / Interleukin-4 | 7.8-500 | Cell culture supernates | KIT11846 |
Human MCP-1/CCL2 | 3.91-250 | Human serum | KIT10134 |
Human TGFB1 (LAP) / TGF-beta 1 | 46.9-3000 | Human serum | KIT10804 |
Human TNF-alpha/TNFA | 23.44-1500 | Cell culture supernates | KIT10602 |
Human TNF-beta | 7.82-500 | Cell culture supernates | KIT10270 |
Mouse IL16 | 5.17-350 | Mouse serum | KIT51303 |
Mouse IL17A/IL-17A/IL17 ELISA | 23.4-1500 | Cell culture supernates | KIT51065 |
Mouse IL-18 | 23.44-1500 | Mouse serum | KIT50073 |
Mouse IL1A / IL-1A / IL-1F1 | 6.56-420 | Mouse serum | KIT50114 |
Influenza H7N9 (A/Anhui/1/2013) HA | 15.6-1000 | Recombinant protein | KIT40103 |
Influenza H7N9 HA | 15.6-1000 | Recombinant protein | KIT40104 |
Influenza H1N1 HA | 18.75-1200 | Recombinant protein | KIT11055 |
Influenza A H1N1 (A/Puerto Rico/8/1934) HA | 46.88-3000 | Recombinant protein | KIT11684 |
Influenza H3N2 Hemagglutinin / HA | 625-40000 | Recombinant protein | KIT11056 |
Influenza A H4N6 (A/mallard/Ohio/657/2002) HA | 62.5-4000 | Recombinant protein | KIT11714 |
Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 | 15.63-1000 | Recombinant protein | KIT11695 |
Human Immunodeficiency Virus type 1 (HIV-1) gp120 | 62.5-4000 | Recombinant protein | KIT11233 |
Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein | 93.75-6000 | Recombinant protein | KIT11049 |