DOT1L Proteins Research Reagents

All DOT1L reagents are produced in house and quality controlled, including 1 DOT1L Protein, 1 DOT1L qPCR. All DOT1L reagents are ready to use.

DOT1L Protein (1)

    DOT1L qPCR Primer (1)

    DOT1L の背景知識

    Histone-lysine N-methyltransferase, H3 lysine-79 specific, also known as Histone H3-K79 methyltransferase, DOT1-like protein, Lysine N-methyltransferase 4 and DOT1L, is a nucleus protein which belongs to theDOT1 family. In contrast to other lysine histone methyltransferase, DOT1L does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones. DOT1L is an histone methyltransferase. It methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA. Methylation of lysine 79 on histone H3 (H3K79) is mediated by DOT1L. It is involved in the regulation of telomeric silencing, development, cell cycle checkpoint and transcription. Mass spectrometry of the DOT1L-containing complex revealed that AF9, ENL and NPM1 were shown to be major DOT1L-interacting proteins. DOT1L might control AF9- and ENL-mediated transcription, regulate RNA processing, and function as a histone chaperone in a NPM1-dependent manner.

    DOT1L の参考文献

    • Nagase T. et al., 2001, DNA Res. 8:85-95.
    • Feng al., 2002, Curr. Biol. 12:1052-8.
    • Dephoure N. et al., 2008, Proc. Natl. Acad. Sci. USA. 105:10762-7.
    • Gauci S. et al., 2009, Anal. Chem. 81:4493-501.
    • Park,G. et al.,  2010, Protein J. 29 (3):213-23. 

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