CLEC4F Proteins, cDNA Clones Research Reagents

All CLEC4F reagents are produced in house and quality controlled, including 29 CLEC4F Gene, 2 CLEC4F Lysate, 2 CLEC4F Protein, 3 CLEC4F qPCR. All CLEC4F reagents are ready to use.

CLEC4F Protein (2)

    CLEC4F cDNA Clone (29)

    NM_001258027.1

    In expression vector

    BC139723

    In expression vector

    In lentiviral vector

    NM_016751.3

    クローニングベクター cDNA 製品

    In lentiviral vector

    NM_053753.1

    クローニングベクター cDNA 製品

    In lentiviral vector

    CLEC4F Lysate (2)

      CLEC4F の背景知識

      CLEC4F, a member of C-type lectins, was firstly purified from rat liver extract with high binding affinity to fucose, galactose and N-acetylgalactosamine, and un-sialylated glucosphingolipids with GalNAc or Gal terminus. However, the biological functions of CLEC4F have not been elucidated. Histochemical staining showed that mouse CLEC4F(mCLEC4F) is only expressed on F4/8+ cells localized in liver, and is undetectable in bone marrow, spleen, lymph nodes, or other tissues in adult mice. However, mCLEC4F is detected in the liver of embryonic day 11.5 (E11.5), which is 1.5 day earlier than the formation of liver (E1) and is 3.5 day earlier than the formation of bone marrow (E15-16). Moreover, recombinant mCLEC4F.Fc binds to alpha-galactoceramide in a Ca++-dependent manner, and both galactose and ceramide can partially inhibit CLEC4F.Fc binding to alpha-galactoceramide. Interestingly, mCLEC4F-deficient (mCEC4F k/o) mice produced far less cytokines than wild type littermates after intravenous injection ofalpha-galactoceramide. This suggests that mCLEC4F is not only a specific marker for Kupffer cells, but is also critical for the presentation of glycolipid antigen to NKT cells.

      CLEC4F の参考文献

      • Shie-Liang Edmond Hsieh, et al. (2009) CLEC4F, A Kupffer Cells Specific Marker, Is Critical for Presentation of Alfa-Galactoceromide to NKT Cells. The Journal of Immunology. 182:78.
      • Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 2004 Jan;36(1):40-5.
      • Bonaldo MF, et al. (1996) Normalization and subtraction: two approaches to facilitate gene discovery. Genome Res. 1996 Sep;6(9):791-806.

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