CD4 Proteins, Antibodies, cDNA Clones, ELISA Kits Research Reagents

All CD4 reagents are produced in house and quality controlled, including 44 CD4 Antibody, 1 CD4 ELISA, 65 CD4 Gene, 1 CD4 IP Kit, 9 CD4 Lysate, 11 CD4 Protein, 3 CD4 qPCR. All CD4 reagents are ready to use.

CD4 Protein (11)

    CD4 Antibody (44)

      CD4 ELISA キット(すぐに使用できます)& ELISA抗体ペアセット(すぐに使用できません)(1)

      CD4 cDNA Clone (65)

      NM_000616.3

      クローニングベクター cDNA 製品

      In lentiviral vector

      NM_013488.2

      クローニングベクター cDNA 製品

      In lentiviral vector

      NM_012705.1

      クローニングベクター cDNA 製品

      In lentiviral vector

      NM_001042662.1

      クローニングベクター cDNA 製品

      In lentiviral vector

      EF492055.1

      クローニングベクター cDNA 製品

      In lentiviral vector

      CD4 Lysate (9)

        CD4 の背景知識

        T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree; however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen-presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen-presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors.

        Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4. Amino acids in both helices found in the cytoplasmic region of VpU in membrane-mimicking detergent micelles experience chemical shift perturbations upon binding to CD4, whereas amino acids between the two helices and at the C-terminus of VpU show no or only small changes, respectively. Paramagnetic spin labels were attached at three sequence positions of a CD4 peptide comprising the transmembrane and cytosolic domains of the receptor. VpU binds to a membrane-proximal region in the cytoplasmic domain of CD4.

        CD4 の参考文献

        • Farrar WL, et al. (1988) Characterization of CD4 glycoprotein determinant-HIV envelope protein interactions: perspectives for analog and vaccine development. Crit Rev Immunol. 8(4): 315-39.
        • Biddison WE, et al. (1989) CD4 expression and function in HLA class II-specific T cells. Immunol Rev. 109: 5-15.
        • Singh SK, et al. (2012) Mapping the interaction between the cytoplasmic domains of HIV-1 viral protein U and human CD4 with NMR spectroscopy. FEBS J. 279(19):3705-14.

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