AHSP Proteins, cDNA Clones Research Reagents

All AHSP reagents are produced in house and quality controlled, including 16 AHSP Gene, 1 AHSP Protein, 1 AHSP qPCR. All AHSP reagents are ready to use.

AHSP Protein (1)

    AHSP cDNA Clone (16)

    BC035842

    クローニングベクター cDNA 製品

    In lentiviral vector

    NM_016633.2

    In expression vector

    AHSP qPCR Primer (1)

    AHSP の背景知識

    AHSP, also known as ERAF, is a conserved mammalian erythroid protein which belongs to the AHSP family. It is expressed in blood and bone marrow. AHSP facilitates the production of Hemoglobin A by stabilizing free α-globin. It rapidly binds to ferrous α with association (k'(AHSP)) and dissociation (k(AHSP)) rate constants of ≈1 μm(-1) s(-1) and .2 s(-1), respectively, at pH 7.4 at 22 ℃. A small slow phase was observed when AHSP binds to excess ferrous αCO. This slow phase appears to be due to cis to trans prolyl isomerization of the Asp(29)-Pro(3) peptide bond in wild-type AHSP because it was absent when αCO was mixed with P3A and P3W AHSP, which are fixed in the trans conformation. This slow phase was also absent when met(Fe(3+))-α reacted with wild-type AHSP, suggesting that met-α is capable of rapidly binding to either Pro(3) conformer. Both wild-type and Pro(3)-substituted AHSPs drive the formation of a met-α hemichrome conformation following binding to either met- or oxy(Fe(2+))-α. The dissociation rate of the met-α·AHSP complex (k(AHSP) ≈ .2 s(-1)) is ~1-fold slower than that for ferrous α·AHSP complexes, resulting in a much higher affinity of AHSP for met-α. Thus, in vivo, AHSP acts as a molecular chaperone by rapidly binding and stabilizing met-α hemichrome folding intermediates. The low rate of met-α dissociation also allows AHSP to have a quality control function by kinetically trapping ferric α and preventing its incorporation into less stable mixed valence Hemoglobin A tetramers. Reduction of AHSP-bound met-α allows more rapid release to β subunits to form stable fully, reduced hemoglobin dimers and tetramers.

    AHSP の参考文献

    • Miele G., et al.,(2001), A novel erythroid-specific marker of transmissible spongiform encephalopathies. Nat. Med. 7:361-364.
    • Zhang Q.-H., et al., (2000), Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.Genome Res. 10:1546-1560.
    • Kihm A.J., et al.,(2002), An abundant erythroid protein that stabilizes free alpha-haemoglobin.Nature 417:758-763.

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