Anti-V5 tag Antibody, Rabbit Polyclonal

Anti-V5 tag Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-V5 tag Antibody, Rabbit Polyclonal
検証済のアプリケーション
WB,IP
特異性
V5 tag
免疫原
A synthetic peptide corresponding to the V5 tag sequence.
調製
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the V5-tag sequence. V5-tag specific IgG was purified by V5-taq affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-V5 tag Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:1000-1:5000
IP 1-4 μl/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-V5 tag Antibody, Rabbit Polyclonal の画像

V5-tag was immunoprecipitated using:

Lane A:0.5 mg his-ARG1-V5 Transfected 293 Cell Lysate

Lane B:0.5 mg V5-ARG1-his Transfected 293 Cell Lysate

Lane C:0.5 mg mFABP4-V5-his Transfected 293 Cell Lysate

Lane D:0.5 mg V5-mFABP4-his Transfected 293 Cell Lysate

Lane E:0.5 mg Negative control

4 μL anti-V5-tag rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-V5-tag rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution

Sample:Recombinant Protein

Lane A: V5-mFABP4-his(30ng)

Lane B: his-ARG1-V5(30ng)

Lane C: mFABP4-V5-his(30ng)

Lane D:Negative control

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-V5 Tag rabbit polyclonal antibody at 1:1000 dilution

Lane A: his-ARG1-V5 Transfected 293 Cell Lysate

Lane B: V5-ARG1-his Transfected 2933 Cell Lysate

Lane C: V5-mFABP4-his Transfected 293 Cell Lysate

Lane D: mFABP4-V5-his Transfected 293 Cell Lysate

Lane E: Negative control

Lysates/proteins at 2 μg per Lane.

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

V5 tag 背景情報

The V5 epitope tag has a 14-amino-acid sequence (GKPIPNPLLGLDST) which is derived from simian parainfluenza virus type 5 (SV5). The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Anti-V5 epitope tag antibody products recognize and detect V5-tagged proteins without a protein-specific antibody or probe in several immunochemical assays such as Western Blotting, ELISAs and immunostaining techniques.
参考文献
  • Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
  • Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.
  • Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.

Standard Antibody Development Service

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Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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