Anti-EpCAM Antibody, Rabbit Polyclonal

Anti-EpCAM Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-EpCAM Antibody, Rabbit Polyclonal
検証済のアプリケーション
ELISA,IHC-P
交差反応
Reacts with: Rat
特異性
Rat EpCAM
免疫原
Recombinant Rat EpCAM / TROP-1 / TACSTD1 protein (Catalog#80306-R08H)
調製
Produced in rabbits immunized with purified, recombinant Rat EpCAM / TROP-1 / TACSTD1 (Catalog#80306-R08H; O55159; Met1-Thr266). EpCAM / TROP-1 / TACSTD1 specific IgG was purified by Rat EpCAM / TROP-1 / TACSTD1 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-EpCAM Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:5000-1:10000
IHC-P 1:10000-1:20000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EpCAM Antibody, Rabbit Polyclonal の画像

Immunochemical staining of rat EPCAM in rat intestine with rabbit polyclonal antibody (1:20000, formalin-fixed paraffin embedded sections).

EpCAM 背景情報

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
完全な名称
epithelial cell adhesion molecule
参考文献
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.

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