Anti-CD73 Antibody

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Anti-CD73 Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-CD73 Antibody
検証済のアプリケーション
WB,ELISA,IHC-P,IP
交差反応
Reacts with: Rat
特異性
Rat CD73
免疫原
Recombinant Rat CD73/NT5E Protein (Catalog#80335-R08H)
調製
Produced in rabbits immunized with purified, recombinant Rat CD73/NT5E (rR CD73/NT5E; Catalog#80335-R08H; Q66HL0; Met1-Lys549). CD73/NT5E specific IgG was purified by Rat CD73/NT5E affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD73 Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:2500-1:10000
IP 0.1-0.5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD73 Antibody (Rabbit Polyclonal 抗体) の画像

Anti-NT5E rabbit polyclonal antibody at 1:500 dilution

Lane A: Rat spleen tissue lysate

Lane B: Rat liver tissue lysate

Lysates/proteins at 30 μg per lane

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:63 kDa

Observed band size:70 kDa

Immunochemical staining of rat NT5E in rat spleen with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of rat NT5E in rat kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

ratNT5E was immunoprecipitated using:

Lane A:0.5 mg A375 Whole Cell Lysate

0.5 µL anti-ratNT5E rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-ratNT5E rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 63 kDa

Observed band size :72 kDa

CD73 背景情報

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
完全な名称
5'-nucleotidase, ecto (CD73)
参考文献
  • Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
  • Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
  • Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
  • Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.

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