SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified

COVID-19 Nucleocapsid Research.
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SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified の製品情報

製品名
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified
検証済のアプリケーション
WB,ELISA
アプリケーションの説明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特異性
2019-nCoV Coronavirus Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV NP Protein (Cat#40143-V08B)
免疫原
Recombinant SARS-CoV-2 / 2019-nCoV Nucleocapsid Protein (Catalog#40588-V08B)
調製
Produced in rabbits immunized with purified, recombinant SARS-CoV-2 / 2019-nCoV Nucleocapsid Protein (Catalog#40588-V08B; YP_009724397.2(335Gly/Ala); Met1-Ala419). The specific IgG was purified by SARS-CoV-2 / 2019-nCoV Nucleocapsid affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified の画像

Anti-SARS-NP rabbit polyclonal antibody at 1:2000 dilution.

Lane A:  SARS-CoV NP Protein (Cat#40143-V08B) (30ng)

Lane B:  SARS-CoV NP Protein (Cat#40143-V08B) (5ng)

Lane C:  SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (10ng)

Lane D:  SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (5ng)

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP  at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified: 別名

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-NCP-CoV Nucleocapsid Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-np Antibody; Anti-nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid 背景情報

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • Potential therapeutic effects of dipyridamole in the severely ill patients with COVID-19
    Author
    Liu, X;Li, Z;Liu, S;Sun, J;Chen, Z;Jiang, M;Zhang, Q;Wei, Y;Wang, X;Huang, YY;Shi, Y;Xu, Y;Xian, H;Bai, F;Ou, C;Xiong, B;Lew, AM;Cui, J;Fang, R;Huang, H;Zhao, J;Hong, X;Zhang, Y;Zhou, F;Luo, HB;
    Year
    2020
    Journal
    Acta Pharm Sin B
    Application
    Foci forming assay
  • COVID-19 Severity Correlates with Weaker T Cell Immunity, Hypercytokinemia and Lung Epithelium Injury
    Author
    Wang, Z;Yang, X;Zhou, Y;Sun, J;Liu, X;Zhang, J;Mei, X;Zhong, J;Zhao, J;Ran, P;
    Year
    2020
    Journal
    Am. J. Respir. Crit. Care Med.

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