Anti-interferon-alpha/IFNA5 Antibody, Rabbit Polyclonal

Anti-interferon-alpha/IFNA5 Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-interferon-alpha/IFNA5 Antibody, Rabbit Polyclonal
検証済のアプリケーション
WB,ELISA,IHC-P,IP
交差反応
Reacts with: Mouse
特異性
Mouse interferon-alpha/IFNA5
免疫原
Recombinant Mouse IFNA5 / IFNaG protein (Catalog#51126-M08H)
調製
Produced in rabbits immunized with purified, recombinant Mouse IFNA5 / IFNaG (Catalog#51126-M08H; NP_034635.2; Met1-Glu189). IFNA5 / IFNaG specific IgG was purified by Mouse IFNA5 / IFNaG affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-interferon-alpha/IFNA5 Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:1000-1:4000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-interferon-alpha/IFNA5 Antibody, Rabbit Polyclonal の画像

Immunochemical staining of mouse IFNA5 in mouse spleen with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).

Mouse IFNA5 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

2 µL anti-Mouse IFNA5 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse IFNA5 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 22 kDa

Observed band size: 22 kDa

Anti-IFNA5 rabbit polyclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:22 kDa

Observed band size:22 kDa

Anti-interferon-alpha/IFNA5 Antibody, Rabbit Polyclonal: 別名

Anti-Ifa5 Antibody; Anti-IFNA5 Antibody

interferon-alpha/IFNA5 背景情報

Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha has antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.
完全な名称
interferon, alpha 5
参考文献
  • Lau JY, et al. (1993) Discrepancy between biochemical and virological responses to interferon-alpha in chronic hepatitis C. Lancet. 342(8881): 1208-9.
  • Kessler DS, et al. (1990) Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator. Genes Dev. 4(10): 1753-65.
  • Gutterman JU. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A. 91(4): 1198-205.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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