Anti-CDC37 Antibody, Rabbit Polyclonal

Anti-CDC37 Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-CDC37 Antibody, Rabbit Polyclonal
検証済のアプリケーション
WB,ELISA,IHC-P,IP
交差反応
Reacts with: Mouse
特異性
Mouse CDC37
免疫原
Recombinant Mouse CDC37 protein (Catalog#50826-M20B)
調製
Produced in rabbits immunized with purified, recombinant Mouse CDC37 (Catalog#50826-M20B; Q61081; Met1-Ala379). CDC37 specific IgG was purified by Mouse CDC37 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CDC37 Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:2500-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CDC37 Antibody, Rabbit Polyclonal の画像

Immunochemical staining of mouse CDC37 in mouse brain with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).
Immunochemical staining of mouse CDC37 in mouse intestine with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).
Immunochemical staining of mouse CDC37 in mouse kidney with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).

Mouse CDC37 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-Mouse CDC37 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse CDC37 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 48 kDa

Anti-CDC37 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:42 kDa

Observed band size:48 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CDC37 Antibody, Rabbit Polyclonal: 別名

Anti-p50 Antibody; Anti-p50Cdc37 Antibody

CDC37 背景情報

CDC37 is a protein that is expressed in proliferative zones during embryonic development and in adult tissues, consistent with a positive role in proliferation and is required for cell division in budding yeast. CDC37 is thought to play an important role in the establishment of signaling pathways controlling cell proliferation through targeting intrinsically unstable oncoprotein kinases such as Cdk-4, Raf-1, and src to the molecular chaperone Hsp90. Decreased Hsp90 expression can reduce the levels of microtubule-associated protein tau, whose overexpression may induce many diseases. CDC37 is considered as a co-chaperone that is classified as Hsp90's accessory proteins. It has been reported that suppression of Cdc37 destabilized tau, leading to its clearance, whereas cdc37 overexpression preserved tau.Cdc37 was found to co-localize with tau in neuronal cells and to physically interact with tau from human brain. Moreover, Cdc37 levels significantly increased with age.
完全な名称
cell division cycle 37
Related Pathways
  • AKT Signaling Pathway
    AKT Signaling Pathway
参考文献
  • Dai K, et al. (1996) P hysical Interaction of Mammalian CDC37 with CDK4. The journal of biological chemistry. 271: 22030-4.
  • Pearl LH, et al. (2005) Hsp90 and Cdc37-a chaperone cancer conspiracy. Current opinion in genetics development. 15 (1): 55-61.
  • Chen GQ, et al. (2002) TNF-Induced Recruitment and Activation of the IKK Complex Require Cdc37 and Hsp90. Molecular cell. 9 (2): 401-10.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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