Recombinant Anti-CD14 Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology

Recombinant Anti-CD14 Antibody, Rabbit Monoclonal の製品情報

製品名
Recombinant Anti-CD14 Antibody, Rabbit Monoclonal
検証済のアプリケーション
FCM,ICC/IF
交差反応
Reacts with: Mouse
特異性
Mouse CD14
免疫原
Recombinant Mouse CD14 protein (Catalog#50023-M08H)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD14 (rM CD14; Catalog#50023-M08H; NP_033971.1; Met1-Pro345).
ソース
Monoclonal Rabbit IgG Clone #503
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-CD14 Antibody, Rabbit Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-CD14 Antibody, Rabbit Monoclonal の画像

Immunofluorescence staining of Mouse CD14 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD14 monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane.
Flow cytometric analysis of Mouse CD14 expression on Raw264.7 cells. Cells were stained with purified anti-Mouse CD14, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-CD14 Antibody, Rabbit Monoclonal: 別名

Anti-CD14 Antibody

CD14 背景情報

The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
完全な名称
CD14 molecule
Related Pathways
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • SD Wright, et al. (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 249 (4975): 1431-3.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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