Recombinant Anti-Argonaute-2 Antibody, Rabbit Monoclonal

RRID Number: AB_2860516
EliteRmab®, manufactured by recombinant technology

Recombinant Anti-Argonaute-2 Antibody, Rabbit Monoclonal の製品情報

製品名
Recombinant Anti-Argonaute-2 Antibody, Rabbit Monoclonal
検証済のアプリケーション
ELISA,IHC-P,IP
交差反応
Reacts with: Mouse
特異性
Mouse Argonaute-2
免疫原
Recombinant Mouse AGO2 protein (Catalog#50683-M07B)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse AGO2 (rM AGO2; Catalog#50683-M07B; Q8CJG0; Met1-Ala860).
ソース
Monoclonal Rabbit IgG Clone #036
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Argonaute-2 Antibody, Rabbit Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Argonaute-2 Antibody, Rabbit Monoclonal の画像

Immunochemical staining of AGO2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

AGO2 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

Lane B:0.5 mg Raw264.7 Whole Cell Lysate

2 µL anti-Mouse AGO2 rabbit monoclonal antibody and 15 μl of  50 %  Protein G agarose.

Primary antibody:

Anti-Mouse AGO2 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 100 kDa

Observed band size: 100 kDa

Immunochemical staining of AGO2 in human brain with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

AGO2 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg MCF-7 Whole Cell Lysate

Lane C:0.5 mg HEK293 Whole Cell Lysate

4 µL anti-AGO2 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-AGO2 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 100 kDa

Observed band size :100 kDa

Recombinant Anti-Argonaute-2 Antibody, Rabbit Monoclonal: 別名

Anti-1110029L17Rik Antibody; Anti-2310051F07Rik Antibody; Anti-Ago2 Antibody; Anti-AI225898 Antibody; Anti-AL022874 Antibody; Anti-AW546247 Antibody; Anti-Eif2c2 Antibody; Anti-ENSMUSG00000072493 Antibody; Anti-Gerp95 Antibody; Anti-Gm10365 Antibody; Anti-KIAA4215 Antibody; Anti-mKIAA4215 Antibody

Argonaute-2 背景情報

Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.
完全な名称
argonaute RISC catalytic component 2
参考文献
  • O'Carroll D, et al. (2007) A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev. 21(16): 1999-2004.
  • Pepper AS, et al. (2009) Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS One. 4(10): e7618.
  • Lima WF, et al. (2009) Binding and cleavage specificities of human Argonaute2. J Biol Chem. 284(38): 26017-28.
  • Adams BD, et al. (2009) Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150(1): 14-23.
  • Salvatore V, et al. (2010) Bacterial expression of mouse argonaute 2 for functional and mutational studies. Int J Mol Sci. 11(2): 745-53.
  • Wilson JA, et al. (2011) Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 85(5): 2342-50.

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