Anti-M13 Antibody (Biotin), Mouse Monoclonal

RRID Number: AB_2857927
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Anti-M13 Antibody (Biotin), Mouse Monoclonal の製品情報

製品名
Anti-M13 Antibody (Biotin), Mouse Monoclonal
検証済のアプリケーション
ELISA
交差反応
Reacts with: other
特異性
other M13
免疫原
M13 Bacteriophage
調製
This product is a recombinant monoclonal antibody expressed from HEK293 cells. Then conjugated with Biotin.
ソース
Monoclonal Mouse IgG1 Clone #MM05
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Biotin
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-M13 Antibody (Biotin), Mouse Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 0.1-0.4 μg/mL
Please Note: Optimal concentrations/dilutions should be determined by the end user.

M13 背景情報

M13 is a filamentous bacteriophage composed of circular single-stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8 and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however, the infection causes turbid plaques in E. coli. It is a non-lytic virus. However, a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single-stranded genome it packages. The general stages to a viral life cycle are infection, replication of the viral genome, assembly of new viral particles, and then release of the progeny particles from the host. Filamentous phage uses a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single-stranded DNA genome to a double-stranded replicative form.
参考文献
  • Messing, J. et al., 1993, Methods Mol. Biol. 23: 9-22.
  • Mori, K. et al., 1996, Antiviral Res. 31 (1-2): 79-86.
  • Sidhu, S.S. et al., 2001, Biomol Eng. 18 (2): 57-63.
  • Sitohy, M. et al., 2006, J Agric Food Chem. 54 (11): 3800-6.
  • Khalil, A.S. et al., 2007, Proc Natl Acad Sci. USA. 104 (12): 4892-7.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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