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TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody


TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody (Rabbit Monoclonal 抗体) の製品情報

TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody
Reacts with: Human
No cross-reactivity with with Mouse TNFR2 (Catalog # 50128-M08H) in ELISA assay
Recombinant Human TNFR2 protein (Catalog#10417-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human TNFRSF1B / CD120b (rh TNFRSF1B; Catalog#10417-H08H; Met 1-Asp 257; NP_001057.1).
Monoclonal Rabbit IgG Clone #R00N6
Protein A
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
< 3 EU/mg
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody (Rabbit Monoclonal 抗体) 検証済のアプリケーション

Block: In a functional ELISA which immobilized recombinant Human TNFR2 (Catalog# 10417-H08H) at 1 μg/mL (100 µL/well) in the plate, the Rabbit anti-Human TNFR2 Monoclonal Antibody (Catalog# 10417-R00N6) can block the binding of 2 μg/mL of biotinylated Human TNFα (Catalog# 10602-HNAE-B) to Human TNFR2 , the EC50 is 2.07 μg/mL .
Neutralization: The neutralization activity of TNFR2/TNFRSF1B Neutralizing Antibody is Measured by its ability to neutralize TNFR2/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 1-3 µg/mL in the presence of 1 μg/mL Recombinant Human TNFR2/TNFRSF1B, 0.25 ng/mL Recombinant Human TNFa, and 1 µg/mL actinomycin D.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody (Rabbit Monoclonal 抗体) の画像

TNFR2/TNFRSF1B-mediated inhibition of cytotoxicity was Neutralized by Human TNFR2 Antibody. Recombinant Human TNFR2/TNFRSF1B (Catalog # 0417-H08H) inhibits Recombinant Human TNFa (Catalog # 10602-HNAE) induced cytotoxicity in the L-929 mouse fibroblast cell line. Inhibition of Recombinant Human TNFa (0.25 ng/mL) activity elicited by Recombinant Human TNFR2/TNFRSF1B (1 µg/mL) is neutralized by increasing concentrations of Human TNFR2/TNFRSF1B Monoclonal Antibody (Catalog # 0417-R00N6). The IC50 is typically 1-3 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNFR2 / CD120b / TNFRSF1B Neutralizing Antibody: 別名

Anti-CD120b Antibody; Anti-p75 Antibody; Anti-p75TNFR Antibody; Anti-TBPII Antibody; Anti-TNF-R-II Antibody; Anti-TNF-R75 Antibody; Anti-TNFBR Antibody; Anti-TNFR1B Antibody; Anti-TNFR2 Antibody; Anti-TNFR80 Antibody


Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD12b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD12b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD12b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD12b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
tumor necrosis factor receptor superfamily, member 1b
  • Komata T, et al. (1999) Association of tumor necrosis factor receptor 2 (TNFR2) polymorphism with susceptibility to systemic lupus erythematosus. Tissue Antigens. 53(6): 527-33.
  • Tsuchiya N, et al. (2001) Analysis of the association of HLA-DRB1, TNFalpha promoter and TNFR2 (TNFRSF1B) polymorphisms with SLE using transmission disequilibrium test. Genes Immun. 2(6): 317-22.
  • Guo G, et al. (1999) Role of TNFR1 and TNFR2 receptors in tubulointerstitial fibrosis of obstructive nephropathy. Am J Physiol. 277(5): 766-72.

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