Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal

Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal
検証済のアプリケーション
WB,ELISA,IHC-P,IP
交差反応
Reacts with: Human
特異性
Human Transferrin Receptor/TFRC
免疫原
Recombinant Human Transferrin Receptor / TFRC / CD71 protein (Catalog#11020-H07H)
調製
Produced in rabbits immunized with purified, recombinant Human Transferrin Receptor / TFRC / CD71 (rh Transferrin Receptor / TFRC / CD71; Catalog#11020-H07H; AAA61153.1; Cys89-Phe 760). Transferrin Receptor / TFRC / CD71 specific IgG was purified by Human Transferrin Receptor / TFRC / CD71 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal の画像

Immunochemical staining of human TFRC in human placenta with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.
Immunochemical staining of human TFRC in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte.
Immunochemical staining of human TFRC in human colon carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to intestinal gland.

Anti-TFRC rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:98 kDa

Observed band size:80 kDa

TFRC was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg K562 Whole Cell Lysate

Lane C:0.5 mg 293T Whole Cell Lysate

Lane D:0.5 mg MCF-7 Whole Cell Lysate

2 µL anti-TFRC rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TFRC rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 84 kDa

Observed band size: 100 kDa

Anti-Transferrin Receptor/TFRC Antibody, Rabbit Polyclonal: 別名

Anti-CD71 Antibody; Anti-p90 Antibody; Anti-T9 Antibody; Anti-TFR Antibody; Anti-TFR1 Antibody; Anti-TR Antibody; Anti-TRFR Antibody

Transferrin Receptor/TFRC 背景情報

Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC / CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a diagnostic tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC / CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC / CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential diagnostic and therapeutic target.
完全な名称
transferrin receptor
参考文献
  • Douabin-Gicquel V., et al., 2001,Hum. Genet. 109:393-401.
  • Ryschich,E. et al., 2004,Eur J Cancer. 40 (9):1418-22.
  • Tosoni D., et al., 2005, Cell 123:875-888.
  • Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
  • Indications for distinct pathogenic mechanisms of asbestos and silica through gene expression profiling of the response of lung epithelial cells
    Author
    Perkins, TN;Peeters, PM;Shukla, A;Arijs, I;Dragon, J;Wouters, EF;Reynaert, NL;Mossman, BT;
    Year
    2014
    Journal
    Hum. Mol. Genet.
    Application
    WB

Standard Antibody Development Service

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Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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